Identification of the interleukin-6/oncostation M response element in the rat tissue inhibitor of metalloproteinases-1 (TIMP-1)Promoter
Identifieur interne : 000E59 ( Main/Exploration ); précédent : 000E58; suivant : 000E60Identification of the interleukin-6/oncostation M response element in the rat tissue inhibitor of metalloproteinases-1 (TIMP-1)Promoter
Auteurs : Marcin Bugno [Allemagne] ; Lutz Graeve [Allemagne] ; Petros Gatsios [Allemagne] ; Aleksander Koj [Allemagne] ; Peter C. Heinrich [Allemagne] ; James Travls [États-Unis] ; Tomasz Kordula [Allemagne]Source :
- Nucleic Acids Research [ 0305-1048 ] ; 1995.
Abstract
The rat tissue inhibitor of metalloproteinases 1 (TIMP-1) gene is expressed in rat hepatocytes, and this expression Is up-regulated by interieukln 6 (IL-6). We report here the cloning of the 5′ flanking region of the rat TIMP-1 gene and identification of an IL-6/oncostatln M (OSM) response element at −64 to −36 which functions In hepatic cells. Within this element we have identified two functional binding sites for transcription factors AP-1 (activatory proteln-1) and STAT (signal transducer and activator of transcription). IL-6/OSM stimulation Induces binding of a protein, identified as STAT3, to the IL-6/0SM response element, while binding of the AP-1 protein was constitutive. Binding sites for both AP-1 and STAT3 are necessary for full responsiveness of the TIMP-1 promoter to IL-6/OSM, as shown by deletion and mutation analysis. Furthermore, the entire IL-6/OSM response element conferred responsiveness onto a heterologous promoter, whereas this has not been observed when AP-1 and STAT elements were separately tested.
Url:
DOI: 10.1093/nar/23.24.5041
Affiliations:
- Allemagne, États-Unis
- District de Cologne, Géorgie (États-Unis), Rhénanie-du-Nord-Westphalie
- Aix-la-Chapelle
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We report here the cloning of the 5′ flanking region of the rat TIMP-1 gene and identification of an IL-6/oncostatln M (OSM) response element at −64 to −36 which functions In hepatic cells. Within this element we have identified two functional binding sites for transcription factors AP-1 (activatory proteln-1) and STAT (signal transducer and activator of transcription). IL-6/OSM stimulation Induces binding of a protein, identified as STAT3, to the IL-6/0SM response element, while binding of the AP-1 protein was constitutive. Binding sites for both AP-1 and STAT3 are necessary for full responsiveness of the TIMP-1 promoter to IL-6/OSM, as shown by deletion and mutation analysis. Furthermore, the entire IL-6/OSM response element conferred responsiveness onto a heterologous promoter, whereas this has not been observed when AP-1 and STAT elements were separately tested.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>États-Unis</li></country><region><li>District de Cologne</li><li>Géorgie (États-Unis)</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Aix-la-Chapelle</li></settlement></list><tree><country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Bugno, Marcin" sort="Bugno, Marcin" uniqKey="Bugno M" first="Marcin" last="Bugno">Marcin Bugno</name></region><name sortKey="Gatsios, Petros" sort="Gatsios, Petros" uniqKey="Gatsios P" first="Petros" last="Gatsios">Petros Gatsios</name><name sortKey="Graeve, Lutz" sort="Graeve, Lutz" uniqKey="Graeve L" first="Lutz" last="Graeve">Lutz Graeve</name><name sortKey="Heinrich, Peter C" sort="Heinrich, Peter C" uniqKey="Heinrich P" first="Peter C." last="Heinrich">Peter C. Heinrich</name><name sortKey="Koj, Aleksander" sort="Koj, Aleksander" uniqKey="Koj A" first="Aleksander" last="Koj">Aleksander Koj</name><name sortKey="Kordula, Tomasz" sort="Kordula, Tomasz" uniqKey="Kordula T" first="Tomasz" last="Kordula">Tomasz Kordula</name></country><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Travls, James" sort="Travls, James" uniqKey="Travls J" first="James" last="Travls">James Travls</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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